So a little update on my grafting and rooting project. The two T bud grafts did not take, they both dried and turned brown, although the seedling did begin to produce callus tissue at the two membranes. i believe since i did not use parafilm that the buds dried out before the graft could take.
All cuttings that were trying to root died none of them grew any roots they produced buds and a few produced actual leaves but then shortly after they turned brown and the leaves wilted and died. i believe that a few things contributed to the unsuccessful outcome of my attempt to root the cuttings, i believe that i did not keep them moist enough to stay alive, though i had them in a propagator with a heat mat and a drip tray with water. i also believe that because i was only watering them with distilled water that this may have contributed to their demise. if i were to do it again i would not use the rooting plugs that came with the propagator i would use wood chips and clay pots as i believe the rooting plugs dried too quickly while i was away at work. with the remaining budwood i put a few buds into a plant tissue culture medium inside of sealed jars and they have been there for about a week now and are not dying and are currently developing "callus" tissue growth as we speak, i did this as i was bored and the budwood had been in the fridge for a few weeks and i thought id try a little experiment.
The medium i used for the plant tissue growth i made at home using.
:Cane Sugar
:Agar Agar powder (found at a health food store, or Asian market in the desert/baking isle its a substitute for gelatin)
:All purpose 10-10-10 fertilizer liquid
:half a multi vitamin
:a 1/4 cup of coconut water
:and 2 tblsp of Clone-X rooting gel
:and distilled water
heated up mixed into the hot distilled water use the agar last. poured into jars and let sit in fridge to solidify and then pressure cooked them to sterilize it all.
Next i decided that i would cut my seedling just below the graft i attempted and regraft it using a cleft graft, and this time i would seal the graft with all purpose parrafin wax i also went to lowes and bought a Dancy tangerine to use as a rootstock, i trimmed the dancy back very heavily when i bought it, i watered it well over night and then trimmed it completely and grafted multiple scions of ugli tangor using cleft graft and the parafin wax (i am still using the flagging tape, im just sealing it with parafin after i have wrapped it). i also ordered some "temple x minneola" budwood and grafted this varriety onto the dancy rootstock with the ugli tangor. The one seedling with an ugli tangor cleft graft and the dancy root stock with ugli tangor and templexmineolla cleft grafts are now in my growing tent under a 150W CFL 6500k bulb and a LED fixture with 12x3WBridgelux18000k 11x3WBridgelux460nm 2x3WBridgelux360nm.
I hope these grafts take this time around. here are some pictures of the dancy after i pruned it but before i grafted it and after i grafted it inside my grow tent.
enjoy
any advice/tips or comments welcomed.