- I don't think there is only one gene (or mutated allele) that confers cold-resistance, rather I think many genes could be involved in a fine regulation system.
- 100 seeds are to few to obtain a "cold-hard" mutant....i mean it's just a matter of statistics.
- If someone would like to find new cold-hardy cultivars, he must find a good selection method.....if one sows a 1.000.000 of Mango's seeds in south florida how can he find the only one cold-resistant? You need a screening that works efficiently (in this case a zone where sometimes temperature falls under 32F), but the same zone has to give the possibility to let Mangos grow until well established...
- Another think is to screen the already present cultivars...I mean, the selection happens without us....it might be that, in some part of the world (North India? or somewhere else, all the Mangos planted die, all apart 1-2 cultivars that were adapted for that not optimal enviroment.....the problem, if there are, is to find them