I pollinate twice sometimes 3 times a day. in summer, if you pollinate a flower at 4 PM, by sunset most female turn to male (the ones you pollinated at 4 PM), then I collect that polled for morning hit. I collect more pollen in morning at 7-10 AM, and pollinate what I can, and container goes back to fridge. there are times I have no pollen but tonnes of female flowers and other times I have pollen but no female flowers. it happens if weather is overcast.
Also, do not collect pollen from male flower if flower seems wilted. that pollen is probably 50% effective.