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But could the stem of an apple or pear be induced to root? What if it had a leaf on it?What type of tissue is typically used for tissue culture propagation?(let's leave patent issues for a separate thread and just talk about what's biologically possible)
https://www.apsnet.org/edcenter/K-12/TeachersGuide/PlantBiotechnology/Pages/Activity5.aspx
The best tissue and simplest it's meristem which contains undifferentiated cells.Virtually all parts of the plant can be used to cloning via callus induction. Even from a single viable cell, but this requires expensive equipment and special techniques. Each plant has a different need and requires chemicals to be used.For instance it's possible to take a lime from store and grow callus from it, then shoots and finally root the shoots. Or use the shoots and micrograft them to a rootstock.
Quote from: pineislander on September 16, 2018, 09:51:23 PMhttps://www.apsnet.org/edcenter/K-12/TeachersGuide/PlantBiotechnology/Pages/Activity5.aspxVery interesting! Amazon has this kit for the reasonable price of $64, but there are zero reviews.Quote from: lebmung on September 17, 2018, 04:43:51 PMThe best tissue and simplest it's meristem which contains undifferentiated cells.Virtually all parts of the plant can be used to cloning via callus induction. Even from a single viable cell, but this requires expensive equipment and special techniques. Each plant has a different need and requires chemicals to be used.For instance it's possible to take a lime from store and grow callus from it, then shoots and finally root the shoots. Or use the shoots and micrograft them to a rootstock.Very very interesting. So this can be done with lime skin? Or which part of a purchased lime? So would this be possible from a mango skin or piece of stem?Who here has experience doing tissue culture? Thanks!
Quote from: TheWaterbug on September 23, 2018, 02:06:34 PMQuote from: pineislander on September 16, 2018, 09:51:23 PMhttps://www.apsnet.org/edcenter/K-12/TeachersGuide/PlantBiotechnology/Pages/Activity5.aspxVery interesting! Amazon has this kit for the reasonable price of $64, but there are zero reviews.Quote from: lebmung on September 17, 2018, 04:43:51 PMThe best tissue and simplest it's meristem which contains undifferentiated cells.Virtually all parts of the plant can be used to cloning via callus induction. Even from a single viable cell, but this requires expensive equipment and special techniques. Each plant has a different need and requires chemicals to be used.For instance it's possible to take a lime from store and grow callus from it, then shoots and finally root the shoots. Or use the shoots and micrograft them to a rootstock.Very very interesting. So this can be done with lime skin? Or which part of a purchased lime? So would this be possible from a mango skin or piece of stem?Who here has experience doing tissue culture? Thanks!I did some tissue culture work as part of my Masters degree project (selected loblolly pine cultivars). I'm surprised to see a kit offered for general use, as what struck me the most about tissue culture production is the absolute need for a sterile environment. It is easiest to keep things sterile in a laboratory, where you can work under dust removing hoods and keep an alcohol flame burning to sterilize your cutting tools. Your agar medium starts out sterile but it's so easy to introduce unwanted organisms to it as you transfer your plant tissues. I had issues with keeping sterile even in a lab - I can't imagine trying to do it at my house. That said, I must say I am intrigued by the kit for sale and might even buy one! :-)
But could the stem of an apple or pear be induced to root? What if it had a leaf on it?Yes and no, in a traditional propagation system using rooting hormones. The stem need to have enough energy to grow roots which it's hardly possible with a stem form store. Stores also spray with fungicides and preservatives which inhibit growth.. From a leaf no.What type of tissue is typically used for tissue culture propagation?The best tissue and simplest it's meristem which contains undifferentiated cells.Virtually all parts of the plant can be used to cloning via callus induction. Even from a single viable cell, but this requires expensive equipment and special techniques. Each plant has a different need and requires chemicals to be used.For instance it's possible to take a lime from store and grow callus form it, then shoots and finally root the shoots. Or use the shoots and micrograft them to a rootstock.
I did some tissue culture work as part of my Masters degree project (selected loblolly pine cultivars). I'm surprised to see a kit offered for general use, as what struck me the most about tissue culture production is the absolute need for a sterile environment. It is easiest to keep things sterile in a laboratory, where you can work under dust removing hoods and keep an alcohol flame burning to sterilize your cutting tools. Your agar medium starts out sterile but it's so easy to introduce unwanted organisms to it as you transfer your plant tissues. I had issues with keeping sterile even in a lab - I can't imagine trying to do it at my house. That said, I must say I am intrigued by the kit for sale and might even buy one! :-)
You picked the hardest to clone. Mangoes are not being TC.
Your aspirations are possible but you lack the know-how.