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Messages - Ilya11

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1
Cold Hardy Citrus / Re: tetraploid seedlings or plants - how to recognize
« on: February 22, 2021, 05:44:48 PM »
First of all we were dealing with poisonous effects of colchicine, that are certainly negligible at the concentrations used for the induction of polyploidy. Also, high osmotic conditions due to the presence of lactose excipient will probably enhance penetration of colchicine into cells

2
Cold Hardy Citrus / Re: cold hardy citrus in full soil in greenhouse
« on: February 21, 2021, 09:46:08 AM »
 I guess that with proper ventilation and shading you can avoid +50C in the greenhouse. Most citruses will not survive such heat.
As to the winter, unheated greenhouse is not particularly effective under long anticyclonic freezes. Kumquats are rather hardy, but only at   vegetation rest. They are late and fruits will be damaged before ripe.

3
Cold Hardy Citrus / Re: tetraploid seedlings or plants - how to recognize
« on: February 20, 2021, 05:14:50 PM »
So, take 5 pills (5 mg), that is what is being used for two and a half  days as a medication, dissolve them in 1 ml of water to have 0.5% of final solution.
 
"Colchicine, in the form of the autumn crocus (Colchicum autumnale), has been used as early as 1500 BC to treat joint swelling.[8] It was approved for medical use in the United States in 1961.[9] It is available as a generic medication in the United Kingdom.[6] In 2017, it was the 201st-most commonly prescribed medication in the United States, with more than two million prescriptions.[10][11"

4
Cold Hardy Citrus / Re: tetraploid seedlings or plants - how to recognize
« on: February 16, 2021, 04:40:08 PM »
Colchicine is not known for its cancerogenicity, it is an authorized medicament to treat gout and several other diseases.

5
Cold Hardy Citrus / Re: F2 citrange winter hardiness trial
« on: February 13, 2021, 10:17:19 AM »
In a summer they are only slightly curvy, while as of today , after  a night at -9.5C they are almost tubular.
I guess this is a sign of cold hardiness adaptation connected with  ability to diminish the surface of water loss.Grapefruit and orange leaves  are not showing this and stay flat until the lethal damage.

6
Cold Hardy Citrus / Re: F2 citrange winter hardiness trial
« on: February 13, 2021, 09:05:44 AM »
This is a matter of numbers.
From my experience if you are able to raise ~200  zygotes from a backcross of F1 plant , there is at least a dozen that are as hardy as an original F1.

7
Citrus General Discussion / Re: monoembryony inheritance question
« on: February 13, 2021, 09:00:28 AM »
Limequat Eustis gave many zygotic seeds.

8
Cold Hardy Citrus / Re: F2 citrange winter hardiness trial
« on: February 10, 2021, 04:17:19 AM »
Kumin,
What were the lows inside this frame?

9
Citrus General Discussion / Re: monoembryony inheritance question
« on: February 03, 2021, 10:59:39 AM »
The varieties that you have used as  mother parent are largely nucellar.
Pollination results in the formation of a zygote secreting  growth factors that induce the formation of  a variable number of nucellar embryos.
This capacity as well as vitality of zygote embryo depends on its genetic composition that comes from both parents.
In many cases nucellar embryos  overgrow the zygote that dies.
That is why the presence/absence of a zygotic seedling and the variable number of nucellar ones  depends on both parents in such crosses.

10
Citrus General Discussion / Re: monoembryony inheritance question
« on: January 30, 2021, 04:04:19 AM »
It served in the past for the preservation of varieties since most of propagation was done by grains.

11
Citrus General Discussion / Re: monoembryony inheritance question
« on: January 29, 2021, 05:22:21 PM »
It seems that there is an idea about monoembryony quality of trees transferring to the offspring when the tree is used as the female parent.  Does  monoembryony transfer in that way?  Any ideas on how it segregates in mono x polyembryonic crosses?

No, polyembryonic feature is not inherited only from a female parent. It is determined by few codominant genes located in nuclear genome.
link
also
more

12
Citrus General Discussion / Re: Non-acidic orange
« on: January 15, 2021, 08:43:43 AM »
Vaniglia is slightly bitter and initially has some acid.
In Maghreb Meski orange landrace is more common. I have one seed grown plant from Tunis  that gives fruits without any acid even in September when they are still green and  small.
Acidless oranges are revolting to  people who expect a normal orange taste, but with the time I  found them rather attractive having a unique, banana like smell.

13
Cold Hardy Citrus / Re: Changsha mandarin opinions
« on: January 12, 2021, 10:15:32 AM »
Thank you. Actually trifoliate leaf form is determined by several codominant genes.

14
Cold Hardy Citrus / Re: Changsha mandarin opinions
« on: January 11, 2021, 12:36:37 PM »
Could you tell us what kind of hybrids you managed to produce with them, please.

15
Cold Hardy Citrus / Re: F2 citrange winter hardiness trial
« on: January 04, 2021, 03:38:58 AM »
Kumin, I wonder if you kept some nucellar seedlings in cold frame  as a control? What is their resistance?

16
Yes, they were frozen, but only for a few hours.

17
mikkel, before freezing the seedlings were acclimated at 12C and Led light 9 hours/day for two weeks. The plants were in plastic container and freezing was first gradual to 0C, equilibration for  3 hours at -3C to let substrat to freeze and than going to -9C with fluctuation on controller of + - 1C

18
My reference control are young seedlings of 5* citrumelo. They lose some leaves but no damage otherwise. The same procedure applied to 5*XTaitian cross gave only 4 survivors out of 150 hybrid seedlings.

19
Actually I have already done this cross. It produced more than 200 hybrid seedlings that in spring were exposed to artifical freezing at -9C for 2 hours. Two dozens survived, three had no damage at all.

20
Cold Hardy Citrus / Re: 17-11 pummelo x citrumelo hybride
« on: December 29, 2020, 04:26:33 AM »
Do we know what happened to this interesting hybrid?
I has not be able to find any further information on it.
In  leaf freeze resistance tests it was shown to have a similiar or even better hardiness than US119.

http://hortsci.ashspublications.org/content/33/4/744.full.pdf

Ilya, the link no longer works.  Do you have this information elsewhere?

https://journals.ashs.org/hortsci/view/journals/hortsci/33/4/article-p744.xml

21
Cold Hardy Citrus / Re: 17-11 pummelo x citrumelo hybride
« on: December 29, 2020, 04:24:57 AM »
Do we know what happened to this interesting hybrid?
I has not be able to find any further information on it.
In  leaf freeze resistance tests it was shown to have a similiar or even better hardiness than US119.

http://hortsci.ashspublications.org/content/33/4/744.full.pdf

Ilya, the link no longer works.  Do you have this information elsewhere?

https://journals.ashs.org/hortsci/view/journals/hortsci/33/4/article-p744.xml

22
Cold Hardy Citrus / Re: Kabosu
« on: December 23, 2020, 04:16:51 AM »
Thank you very much for a detailed report. I was never aware of sweet taste of internal membranes of Kabosu. I also noticed that older leaves are yellow now. Is it a regular feature of this variety?

23
Cold Hardy Citrus / Re: Long term cold hardy citrus breeding project
« on: December 17, 2020, 04:07:56 AM »
If you select out of large hybrid populations, there are sufficient number of hardy plants. Absence of trifoliate taste can be also assured. But of course for sweetness, size of fruits etc., you need to wait for plant maturity. I have a dozen of plants that produce fruits, but have not yet attained my goal.

24
Cold Hardy Citrus / Re: Identify this fruit?
« on: December 16, 2020, 04:24:33 AM »
In order to get its seeds it is necessary to artificially pollinate with a lot of foreign pollen. The best is that of Meyer. The resulting seeds are nucellar  with many embryos. 

25
Cold Hardy Citrus / Re: Identify this fruit?
« on: December 15, 2020, 04:38:34 PM »
Here is a second harvest of one of the  Morton seedlings from 2011 fruits described above


 




Most fruits are round without distortions, but this variety is a magnet for citrus bud mite giving occasionally very strange forms






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