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Thoughts on oryzalin experiments especially with precocious plants

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vnomonee:
This winter, while outdoor gardening stops, I’m brainstorming a series of indoor experiments using oryzalin as a safer alternative to colchicine for inducing polyploidy. I’m especially interested in seeing how different species respond, both in growth and eventual fruit quality.

Some ideas working with seeds that I have or need to source:
Hindsii kumquat (my tree died from root rot this spring)
Fast flowering trifoliate,
Afganski pomegranate,
A large-fruit pawpaw (not really precocious but I don't see polyploid pawpaw varieties in the trade)
As well as doing targeted bud treatments on my Passiflora incarnata × edulis hybrid cuttings.

My goals are to explore whether polyploid lines can give improvements like thicker foliage, increased vigor, larger fruit, or in the Passiflora hybrid potential restoration of fertility where sterility is an issue. I think these trials could lead to some unusual phenotypes, maybe even new breeding material and a good way to kill time when it's freezing out.

a_Vivaldi:
I don't know about in the other plans but I'm Passiflora tetraploidy is useful. The flowers tend to be larger and the stems and leaves are more robust. The fruit I don't think are any bigger but it's possible the arils are a little bigger and so are more juicy.

The biggest benefit though is better fertility, especially with hybrids. Unlike Citrus, Passiflora is often not so tolerant of crosses, with high rates is partial or complete sterility in hybrids. Tetraploid hybrids are usually not as bad for infertility.

I myself recently treated some seedlings, not sure yet if the treatment worked.

vnomonee:
Cool, would love to see the results on those seeds when you notice anything.
If you haven't seen this blog already https://gardenscientist.wordpress.com/2014/10/03/polyploid-update-fall-2014/
could be useful

usirius:
Interesting approach; I've also studied and heard quite a bit about this. There are several studies freely available online. Gibberish and colchicine can positively contribute to the multiplication of the chromosome set, which can then partially enable the pollination of incompatible species due to unequal chromosome numbers. But where does one obtain colchicine? Well, one could collect autumn crocus and make an extract, which naturally contains colchicine, but certainly not only that... Does anyone have any experience or ideas on this?

Walt:
Last I knew Carolina Biological Supply sold colchicine.  It is very expensive,  Oryzalin is much cheaper. last I bought some.  "oryzalin as a safer alternative to colchicine"   That is what the litterature says.  But I wonder if it is just less studied.  I think that anything that can stop cell division without stopping chromosome division should be treated as a poison.  That said, either can be safely used.  Get none on you.  If used inside, have an open window with a fan blowing out to keep any fumes from going your way.  Better still, use outside with your back to the wind.  Store it where no one can get to it, in a sealed container.

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