Citrus > Citrus General Discussion

Thoughts on oryzalin experiments especially with precocious plants

<< < (2/3) > >>

citrange:
Some years ago I bought some Autumn Crocus bulbs (=colchicine) and mashed them up in our kitchen liquidiser. I did then get a bit scared about this having read how dangerous colchicine is, so I washed out the equipment several times and never told my wife!
Anyway, I mixed this into a seed compost and planted a load of Poncirus seeds hoping to get some variegated seedlings or other genetic modification. They were all perfectly normal, so I never tried the experiment again. Of course, I've no idea how much colchicine was actually present or how much might be needed to affect the germinating seeds.

vnomonee:
Without some lab equipment I wouldn't try to extract it from crocus. It's toxic and also a carcinogen! It looks like repeated treatments would need to be done, not just a simple soaking.
Oryzalin is not as dangerous, but I am having trouble finding any suppliers that will ship to NJ.

Southern Ag Surflan As runs about $20 shipped for an 8oz bottle if the supplier can ship to your state.

Ilya11:
Colchicine is an officially approved drug for the gout treatment.
link
The belief of its extremely  high toxicity for the humans is an over-exaggeration.

tritonus:
I'm planning to do similar experiments as you vnomonee with Loquats.
Oryzalin is hard to get for regular people where I live, but luckily I know a biology professor who can order it for me.

Would I spray the aqeous solution on the apical bud of the young seedling, or submerge it?

I'm getting a microscope as well, hoping that I can observe polypoidy through stomata size increase. I think I won't manage to count chromosomes, as it looks a bit tricky for a noob like me. Does anyone have other ideas on how to determine chromosome count?



Ahuacatl:
Other indicators are stomata size, pollen grain size and flower size.  The most useful
Is pollen grain size since it will tell you if the plant is making large polen grains and how fertile it is.

Navigation

[0] Message Index

[#] Next page

[*] Previous page

Go to full version