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Redomestication of Cold-Resistant Bananas

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Lauta_hibrid:
For now I have few genetic resources, my other bananas are still missing to give flowers, so I will use these. And about Cavendish, zebrina muse and velutina muse: with the cold my Cavendish dies the pseudotayo, zebra too and velutina muse too... although in hesitation it has remained firm this winter and just the spring is already making flowers. To my experience in my climate velutina has resisted more and gave flower fast, pires corra. A little above my waist it already bloomed. I like that since the short cycle allows that if the pseudo stem is burned that does not harm the recovery and formation of fruit. But I'm very interested in Musa manii, do you have seeds of that variety? My Citrus hybridization project is already in its flowering stage after 6 years of work, so I have to focus on my next project. I also have Musa ornata, that one keeps the pseudo stem in the same conditions, as do Mysore and Bluggoe. Do you have any idea the cold resistance of ornata?
By the way, I was reading a paper that talks about the origin of ABB bananas and their genetic complication in the analyzes. And there they clarify that the BBBs are actually ABB but with a retro crossing with balbisiana, so the analyzes did not make their origin clear. I already suspected it since there was no parthenocarpal balbisiana.

JSea:
There's from memory a few forms of velutina that exist (e.g. Musa velutina subsp. markkuana), and some of them have possibly hybridized with Musa ornata and similar species before, so cold tolerance can vary somewhat I think.

I don't have seeds of Musa mannii, my 2 plants are not very large yet (I think they are slower growing than Musa velutina, in exchange for the extra cold tolerance). I should have seeds in a few years :) But also the plants are getting established well now, and I'll repot them any week now and hopefully get a good Summer of growth.
I should also note I was never able to sprout a single Musa velutina despite trying maybe 3-4 times with hundreds of seeds (some straight out of the fruit), but I sprouted Musa mannii twice with two attempts (once with fresh fruit, another time with dry seeds).

Musa ornata I'm not too sure on the cold tolerance of. It does hybridize freely so it may not always be pure and may vary. I wouldn't class it as a cold tolerant banana though I think, not like M. sikkimensis / M. basjoo / M. yunnanensis anyway.

I would be interested to read that paper about the BBB varieties, as I did wonder how it's possible that they existed.

Lauta_hibrid:
Unraveling the complex story of intergenomic recombination in ABB allotriploid bananas
https://www.biorxiv.org/content/10.1101/830059v1.full

Lauta_hibrid:
I'm going to show you my latest attempt at crossing Asian hornet (Vespa velutina) with Bluggoe. Luckily, I found a flowering plant in the center of my city. As you can see in the first photo, the stamens were opening, revealing that there was something inside. To properly remove the small amount of pollen found on a stamen, you should gently scrape it with a smooth surface like a knife. The friction lifts the two longitudinal flaps that cover the pollen area, thus recovering some pollen. To be fertile, it must be white, unlike citrus, which is orange-yellow. Its appearance is not powdery. I picked the Asian hornet with the inflorescence still having its first two bracts closed. This is because Asian hornet flowers are hermaphroditic and tend to release pollen before opening, which would ruin our plans. I moved the bracts back and carefully removed the flower petals and stamens. Keep in mind that you should check the stamen by gently pricking it with your fingertips to see if any pollen remains. If pollen is released, the stigma must be removed to avoid contaminating the next flower, as they are very close together. Then I proceeded to check the bluggoe flowers and rubbed them when I saw the stamen still closed by those little sacs (which I don't know the name of). I saw plenty of white pollen, so I pollinated them. From the last three flowers, I removed the stamens, style, and stigma to see if the ovary develops or not; that will tell me if fertilization occurred. The last thing is to cover it with a piece of paper like a hood so that no insect dares to pollinate it. We'll see what happens.
The only bibliography I found on the use of triploid pollen in diploid birds was with Cavendish, and according to the report, all the offspring were diploid. This reflects that their pollen only fertilized when it was haploid, so this could give combinations like: VV x ABB = VA, VB, or VB

Keep in mind that only Acuminata (genome A) has parthenocarpy genes, so many may produce seeds. I hope some are easy to spot (by morphology or height, and are triploid).

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