Citrus > Cold Hardy Citrus
Making new periclinal chimeras like Prague
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Walt:
A new way to make periclinal chimeras in citrus is given here.
日 植 病 報 60: 82-88 (1994)
Ann. Phytopath. Soc. Japan 60: 82-88 (1994)
OK, 1994 isn;t new but it is new to mr, The procass was developed to give disease resistanc in citrus by giving a good variety a coating of a disease resistant variety, But it seems to me it might work on making good citrus varioties a coating of a cold tolerant variety like ponciris, The publication says 1/6 of plants made this way were periclinal chimeras,
One would need to use a good varity of citrus that has nucelar seeds, No problem finding those,
I don't expect the rresulting plants to be hardy in one 6 where I live, But maybe zone 7 or 8? Maybe anywhere PPrague is hardy?
poncirsguy:
My area is predicted to reach a solid zone u by 2050. My wife and I will have a combined age of 194 years, so we won't be holding are breaths.
Akebia:
Several years ago I looked through the literature on chimera synthesis, and this technique appears to be the most efficient method that isn't in vitro. Its more efficient than a lot of the in vitro methods. The Japanese have done a lot of work in this area. I think its a very promising technique. It consists of approach grafting two seedlings, decapitating the shoot tips, and then further cutting just one of the seedlings at like a 45 degree angle. Then you apply a plant hormone blend and put parafilm over them. To select out chimeras you look for the shoots that are slowly growing. I guess with citrus you could say its a 1/12 success rate instead of a 1/6--given that half would have poncirus flesh. But still that's amazing to do in just one generation and isn't a ton of work either. Of course for further breeding a chimera is a dead end.
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