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Messages - usirius

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1
Cold Hardy Citrus / Re: Percentage of zygosity HRS899
« on: November 25, 2024, 05:10:55 PM »
I don't have an HRS899 A, but I do have an HRS899 O/Q, probably the only one in existence, it flowers and fruits reliably and the seedlings are mostly zygotic, which can be easily seen from the different appearance.

2
Tropical Fruit Discussion / Re: Grafting Che to Mulberry
« on: November 13, 2024, 05:31:42 PM »
Anyone ever try grafting mulberry to Che?

I will try it this winter.

3
Citrus General Discussion / Re: Poncirus polyandra turning yellow
« on: November 13, 2024, 05:15:57 PM »
Incidentally, as has been mentioned, Poncirus polyandra is more frost-hardy; for years I have been cultivating it outdoors with very little winter protection.

I think this is the first time I've seen anyone state what polyandra's hardiness was.

I estimate that it survived -12 °C and showed no signs of frost damage.

Hello usirius,

great that you have both forms. I've only ever heard that there are 2 forms and didn't know which form mine belonged to
I think I have the one with the smaller leaves. It also seems to be more like the one in the species description.

At the beginning I had a plant outside, but it froze back at -6°C, since then I've had it in a pot. How much has it survived with you?

In the photos it looks as if the form with the larger leaves occasionally develops monofoliate leaves. Is this the case or have the smaller leaves fallen off?
Have your plants already flowered? It has already flowered in Oer-Erkenschwick, but I haven't read anything about fruit yet. One of my plants is descended from this flowering one, but there have been no flowers here yet.

Thank you!

The leafes are in geeneral trifoliat but this year the snails have increased in number and are eating the leaves on the young shoots, so that often one or two leaves are missing, then they appear bi- or monofoliate. Mine showed no damage, even in very extreme winters. I think you have a different variety or one from a different source if it has suffered major damage at -6°C. - They are not very fertile, but every now and then they keep a fruit, which they lose unripe in late autumn.

4
Cold Hardy Citrus / Re: Ponciruslike fruit comparison
« on: November 13, 2024, 09:36:25 AM »
usirius,
perhaps your ponсirus does not produce fruits from summer flowering, but this is not the case everywhere. This may be due to the weather or the characteristics of the clone. Skandiberg previously wrote that the Bayush clone now has unripe green fruits at the top of the tree. I also noted this on another ponсirus and showed a photo. This property of ponсirus has been discussed on forums more than once. Unripe green fruits on the tree certainly affect winter hardiness. And for a hybrid of pontirus with other citruses, it is better not to have late flowering. The question is whether there are clones without summer flowering, or all mature strong trees in good conditions re-bloom in summer.

On my poncirus alsot these fruits o not manage to ripen. But it is sufficiant hardy nevertheless also on such twigs!

5
Citrus General Discussion / Re: Poncirus polyandra turning yellow
« on: November 13, 2024, 09:28:01 AM »
Well, it's not such a secret. I know the young man personally who visited the native region of Poncirus Polyandra in China over 20 years ago. He got some material from there, which he gave to a citrus friend to save and propagate. From there, some of the material ended up in the Damrstadt Botanical Garden (Germany). There are two types of Poncirus polyandra, which differ significantly in terms of leaf size and appearance. To illustrate this, I photographed both types side by side. Incidentally, as has been mentioned, Poncirus polyandra is more frost-hardy; for years I have been cultivating it outdoors with very little winter protection.


The origin must remain a secret :) but there are now several collectors who have them and a botanical garden in Germany also got them this way.
So far all the flowers are sterile and no fruit has formed




6
Cold Hardy Citrus / Re: A non bitter larger Poncirus?
« on: November 13, 2024, 02:22:30 AM »
I gave some 'Bajusz' fruits to the owner of tropusikert.hu. He says 'Bajusz' is better than what remembers of the selections he sells/sold.

Interesting!

I have checked his store and found he is offering also Poncirus trifoliata  ’Hazai óriás’ (what means 'Domestic giant')

He writes about this PT selection:
 -giant garden lemon . Fully winter-hardy, selected down to -28 degrees, very high-yielding variety. Not bitter! Even small seedlings can withstand -25 degrees

Are you able to get some fuits of it for comparison?

7
Cold Hardy Citrus / Re: Ponciruslike fruit comparison
« on: November 13, 2024, 02:13:42 AM »
It can actually happen that Poncirus blooms again on individual branches in late summer, but these fruit buds do not manage to ripen. I have observed this in some years. It may be related to the weather in summer. - It is simply an early opening of the buds that were formed for the next season. But it does not harm the winter hardiness in any way, as the opening does not happen during the dormant period but long before the plant goes into dormancy. The winter hardiness is not affected by this.

Much worse is if Poncirus wakes up too early when temperatures are too warm in early spring, followed by frosts. Poncirus is particularly at risk in a location facing south, perhaps in front of a wall, where the temperatures warm everything up too quickly, for example due to the winter sun, and the sap and shoots start to sprout. A frost can then damage it severely, sometimes to the point of complete death.

8
Citrus General Discussion / Re: Graft method with leaves attached
« on: November 12, 2024, 01:37:48 AM »
Thank you hardyvermont for introducing this rather unusual grafting method!

I have used this method successfully. Sometimes I cut the leaves in half or down to the winged petiole if there are several. I think that the remaining leaves, if they are protected from damage, support the plant's growth and subsequent growth better than if they are removed.

However, if I only graft one bud, I remove the leaf completely.

9
Cold Hardy Citrus / Re: Ponciruslike fruit comparison
« on: November 12, 2024, 01:27:05 AM »
In order to give a more complete picture of the fruit comparison described here with Poncirus-like fruits, I would like to give two more short feedbacks, which come from two citrus friends who have also tasted some of the fruits mentioned by Skandiberg.


Feedback from A)

All in all there is only one result: your new HRS fruit, around only 5 seeds, sweet, sour, aromatic Bajusz no comparison Poncirus # 7 new fruit over d > 6cm, juice sour but also no comparison. "


Feedback from B)
 
HRS 899 O/Q was very good. I could drink the juice neat. I also found Hybrid #8 very good. I liked the aroma of HRS 899 O/Q a little better because of the mandarin notes, but Hybrid #8 didn't have a bad aftertaste either. I found Hybrid #7 disappointing, but it was as I remembered it. It's a kind of milder Poncirus, which is good in that respect, but it's clearly behind the others. My relatively negative assessment of Hybrid #7 was also due to the fact that I had tasted my PT #1 at the same time, which at first glance just tasted good, but then had a slightly not entirely pleasant aftertaste, but was definitely better than #7. Bajusz was just as good as my PT #1. So my conclusion about the fruit is: HRS 899 Q/O is the plant of choice for the most important breeding projects. Hybrid #8 comes right after that, closely followed by my PT #1

(Note: His PT #1 he discovered from a bunch of purchased Poncirus seedlings, which produces significant better fruits than common PT)

10
Cold Hardy Citrus / Re: Ponciruslike fruit comparison
« on: November 09, 2024, 10:51:17 AM »
Thank you Jibro for your post!

es, it is absolutely true that the presence of a navel does not necessarily indicate hybridity. Thanks also for the link to the description of individual Poncirus varieties with partial navel formation on the fruit!

What I notice, however, is that even with these Poncirus varieties, the navel is only partially present and not so strongly developed.

In the Poncirus from Woodlanders that is to be identified, the navel is present on almost all fruits and it is very large and clearly developed.

Another striking feature is that the fruits are now significantly greenish, whereas those of other Poncirus and Poncirus-like ones are already orange in color.

You cannot see anything significantly different about the leaves compared to Poncirus leaves when you take them into account, but that is also the case with my hRS8999 O/Q hybrid.

I think I have to do some further research on other criteria, such as the flowers.


Navel on poncirus fruits is not that uncommon and some poncirus clones have fruits with navel regularly, you can see it on some US trifoliates: https://citrusvariety.ucr.edu/crc3345
https://citrusvariety.ucr.edu/crc2862
https://citrusvariety.ucr.edu/crc3217
https://citrusvariety.ucr.edu/crc3206
So navel alone is not an indication that it is a hybrid, in my first trifoliate test a few years ago I also had 2 fruits with navel.

11
Cold Hardy Citrus / Re: Ponciruslike fruit comparison
« on: November 08, 2024, 06:16:23 PM »
I'm really happy about your comment. Do you know why? I got this Poncirus from a citrus friend to test. He bought the plant many years ago from Woodlanders Inc. under the name "Poncirus trifoliata". He asked me to tell him my impression of whether it was Poncirus. So, when I compare the fruit with the fruit of my neighbor's Poncirus, one of the externally noticeable differences is actually the navel on the fruit, plus the smaller size and the greener color, which may indicate that it is not yet fully ripe. I could imagine that this Woodlanders Poncirus is not a pure Poncirus, but perhaps an F2 generation of a Poncirus-Citrus hybrid, or a backcross of a Poncirus-Citrus hybrid with Poncirus. What do you think? Or do you think that is a pure Poncirus?

Attached is a picture with several fruits of the Woodlanders Poncirus compared to my neighbor's Poncirus.




12
Cold Hardy Citrus / Re: Ponciruslike fruit comparison
« on: November 08, 2024, 12:32:29 PM »
Hello Skandiberg, BorisR and Jibro,

thanks for your contributions and thoughts!

I also made a comparison, although due to time constraints I did not evaluate it in such detail.
I have mostly fruits of the same varieties, which all appear quite poncirus-like, both in their appearance (i.e. trifoliate leaves, deciduous in winter, very thorny growth, fruits with velvety hairs, and what is also a very important criterion, frost-hardy to at least -20°C, i.e. a cultivation category in which the previously known hybrids, which are of course much more usable, cannot keep up.


Fruit varieties


Fruit varieties  - sliced ​​fruits


Fruit varieties  - sliced ​​fruit - close-up


However, due to the large number of varieties and lack of time, only one fruit at a time. At some point during the tasting, the impression was probably distorted, because the foul, bitter pine-like aroma and the stickiness of the previous tastings - once you have it in your mouth, it is not so easy to eliminate for subsequent tastings....

I used a slightly different rating system, namely a gradation from 1 - 5 for various criteria that I consider valuable in terms of utilization and for a possible further breeding as a parent. The grade 1 corresponds to the worst value for a criterion, and the grade 5 to the best value for a criterion for Poncirus species from my breeding point of view.



But I also think that comparability is difficult due to different locations and also harvest maturity times and with fruits from different positions in one plant, and in one case the quality or aroma has certainly already suffered due to over-cultivation.

13
Hello, I'm interested in citrus precocity. I don't mind the trait is recessive. Are there any places with seeds or cuttings? Are there aternatively any other precocious citruses?

I currently have few fruits and seeds available from an improve hardy poncirus hybrid and also from a good and hardy precocious poncirus hybrid, 1 fruit of each variety 2,50 EUR

14
Cold Hardy Citrus / Re: A non bitter larger Poncirus?
« on: November 02, 2024, 07:37:14 AM »
@Bussone:  Thank you for this interesting article and for your thoughts on the selection of Poncirus trifoliata for its positive properties as a rootstock. Hybrids of Poncirus trifoliata and Citrus were bred with the same aim, namely to serve as a rootstock with better properties than pure Poncirus trifoliata, and not with a view to the usability of the fruit.

This could well explain the poor fruit quality of commonly available Poncirus trifoliata and its hybrids, and also that there can also be Poncirus trifoliata with better fruit properties outside of normal citrus regions.

So folks, be brave and taste the fruits of the Poncirus trifoliata in your area without any preconceptions, if you haven't already done so! Maybe you'll make an interesting discovery, like I did recently with my Flying Dragon, which I've paid little attention to until now, but which has been growing in my garden for over 20 years and bears perfectly usable fruits with hardly any bitterness.

Wow.

Stupid or not, it was worth asking that question.

Boris and Bussone,

Thank you for the article and the additional information. I knew of the variability of PT but this is new to me. I thought all Poncirus clones were superior to any of the hybrids in hardiness boosting, just by a different margin. And so it seems they aren't. Thank you for pointing it out.

This is an interesting article.
https://www.osti.gov/servlets/purl/1815977

There are two primary takeaways, I think.

1. Poncirus isn't just cold-hardy. It's also really disease-hardy, and its genome is much more active on the cold-hardiness and disease-resistance fronts than is typical of citrus. (This is why it's beloved as a rootstock)
2. The US poncirus selections are not diverse.

Thus, it's conceivable that our image of poncirus as sticky, foul-tasting mess may just be a selection of the items the USDA saw promising as rootstock after a few gatherings, selected with flavor entirely as an after-thought. Non-US poncirus examples may have very different offerings and provide different experiences.

15
Cold Hardy Citrus / Re: A non bitter larger Poncirus?
« on: October 30, 2024, 06:08:54 AM »
When tasting the fruits of the Flying Dragon tree and HRS899 O/Q, I made a completely unexpected discovery.

But let me first tell som background:

I have planted a Flying Dragon tree about 20 years ago, which was suffering for a long time, then slowly started to grow, but in the meantime was overtaken by the Trachycarpus fortunei that surround it, so that it is now in their shadow. I have never tasted its fruits.

In addition, as posted here in the forum some time ago, I have a seedling of the Hybrid HRS899 (Poncirus x Chasgha mandarin), which has survived many frosts down to -17°C for over 20 years without any damage, and a graft of the same survived -20°C and in following night -19°C this winter without any damage. I once received this seedling and other siblings of this seedling for testing from the citrus breeder and citrus gardener Bernhard Voß, who had received the seeds from the Florida Arboretum. But only this seedling survived and after tasting the fruits, several citrus friends classified them as interesting and better than Poncirus trifoliata or Poncirus Hybrid #7.

Now this year - for whatever reason - a surprise came:

I tasted both fruits together and, lo and behold, the fruits of this Flying Dragon are quite pleasant to taste! The skin also does not have any strong unpleasant aromas typical of Poncirus. Of course, the skin of my HRS899 tasted better in the tasting, but in terms of the quality of the flesh, in my opinion, both are not far apart. However, I only tasted one HRS899 fruit because I have a lot of requests, so I took the one that was lying on the ground and was the very first to fall off three weeks ago. Could this be the reason why the aroma, especially the mandarin note, is not so exceptionally pronounced?

As I said, I had never tasted the fruits of my Flying Dragon before, so I cannot say how they would taste in a normal hot, dry summer and of course without sun protection from palm leaves.

But let me put one thought out there:

Is the reason for these two fruit tasting results the rainy year, because here it rained extraordinarily much from spring through summer and into autumn, and the sun shone correspondingly less.

Perhaps a lack of sunlight reduces both the good and the unpleasant aromas? Could that be the case? What do you think?Pleas let me know your experiences and your thoughts.

-----------------------------------------

Here are two more pictures showing the sliced ​​fruits that we tasted. You can clearly see the number of seeds, the thickness of the fruit skin and also the appearance of the juice.


Fruit of my HRS899 O/Q


Fruit of my Flying Dragon


16
Cold Hardy Citrus / Re: A non bitter larger Poncirus?
« on: October 29, 2024, 09:25:43 AM »
1.262 / 5.000
Hello Skadiberg,

Thank you for the further research on the plant where these fruits come from. Also thank you for the research on the minimum winter temperatures. That sounds good at first glance, but the following must be considered: Did the winter temperatures prevail at the exact location where this tree stands? You certainly know that a microclimate can be very different to the climate officially measured at a measuring station, for example if the location is in a town and even in a courtyard where a plant survives. It must be remembered that the height above ground also has a strong influence on the measurement of the temperature. And it must also be considered whether the measured low temperatures occurred after previous mild temperature periods, or during the absolute winter dormancy in a period of frost that has already lasted for a long time. Speaking of frost resistance: Poncirus trifoliata can die completely in February if mild temperatures precede it and then frosts of -6°C follow, as happened a few years ago in Italy. After a long period of mild temperatures during the winter, citrus vrelatives wake up and as soon as the sap flow begins, their winter hardiness decreases rapidly.


Yes, exactly.

Whatever it is, 'Bajusz' likely sailed through -19/-20⁰C at least two times in its lifetime. I think that is decent but still not uncommon for a PT, but any hybrid can be proud of that.

17
Cold Hardy Citrus / Re: A non bitter larger Poncirus?
« on: October 28, 2024, 09:18:12 AM »
@Till: Thank you for sharing your experiences when tasting fruits of Skandiberg! Maybe I can make it possible to to update your remembrance!

Skandiberg sent me some fruits of a better Poncirus a few days ago. Here my review:

The fruits were very juicy. That is not untypical for Poncirus. But the pulp contained less fiber material than other Ponciruses. After staining only seeds, hardly any pulp remained. The smell of the unopend fruits was very pleasant. Also not untypical. But the smell of the opened fruits was also quite pleasant, that is to say much less resinuous than common Poncirus. I tasted the fresh fruits. They contained a normal amount of sticky substance and had the typcial tan flavour that is the main obstacle for enjoying Poncirus fruits. Again, the tan flavour was much less pronounced than in normal Poncirus so that I could eat the pulp without selfcontrol. The peel was horribly bitter as any other Poncirus peel is.
I let the juicy stand for half a day as I always do with Poncirus juice so that the sticky substance falls to the bottom of the glas and the resiuous / tan flavour fades away. Then I made lemonade from the juice. Such a lemonade is usually very tasty no matter what Poncirus types you use. In this case, it was outstanding tasty without any bitterness, just a very good and balanced citrus taste (lemony with orange subtones).
I really recommend this Poncirus type for propagation and cuisine use. I can directly compare it to Swamp Lemon (= Nikita) which I processes a few days before. Skandibergs Poncirus is significantly better. It contains less of the typical Poncirus off-flavours (the resinuous ones). The lemonade from Swamp Lemon was also tasty. My children also enjoyed it. But they made an interesting comment: "There is something in it, I don't know what, that is strange. But still very tasty." This hard to describe something (probably a weak reflex of some bitterness or resin) is totally absent in the lemonade from the fruits sent to me by Skandiberg. But the greatest difference can be observed when you compare fresh fruits. Swamp Lemon is closer to normal Poncirus.

I have never eaten Poncirus+ fruits. But in my personal ranking Till #1 (a variety discovered by myself) is the very best without any off-flavours in fresh fruits (at least in one, the first, year), followed by Skandibergs fruits, follwed by Swamp Lemon and then a variety of Mainz botanical garden (the left one in the arboretum). I should eat fruits of Changsha x Poncirus F2 from Usirius and PT#7 again to update my memories. So far as I remember these fruits might compete with the fruits of Skandiberg. The unique property of Usirius hybrid is a mandarine flavour, less acids and less sticky substance. PT#7 has just Poncirus taste but is milder, less sour and containes less sticky substance. Both hybrids are said to be as hardy as Poncirus.

18

Does anyone else grow Kyoho or ruby Roman? Has it ever produced the golf ball sized grapes? Do they taste the same as the ones in Japan?

I am growing Kyoho and the fruits are really extraordinary karge. Kyoho beeds  a long warm season  to make sure rhat the fruits grow karfe,  get fully colored and mature snd sweet.

I bought my Kyoho from
 a nursery which is spezialzed on propagating and selling only grape vines..

19
Temperate Fruit Buy, Sell, & Trade / Re: Free Asiminia triloba seeds
« on: November 19, 2020, 01:56:48 PM »
I have many seeds
I am always trying to have seeds of different area's to widen the gene pool (In woods wild planting)
Maybe I could trade for different pawpaw seeds as someone could use these,
and do not want to ask of something I already have, (My Mom taught me that)

Would you want that Take some of my seeds spread them where your family lives, and send me some of yours from Michigan ?
(or anyone else send me some from there area for some of mine)

From a good friend I got few pawpaw seeds from the area of Сочи - fruits are large and tasty. I am looking forward to grow them succesful.

20
Temperate Fruit Buy, Sell, & Trade / Re: WTB sweet cherry scions
« on: November 19, 2020, 05:55:28 AM »
Hi, I can send you scions in early spring from a yummy wild sweet black cherry and from "Hedelfinger" a well tasting dark red sweet cherry and maybe also one or two unnamed varieties, if you like them also. In case of interest send me a PN.

21
Temperate Fruit Discussion / Re: Cherimoya/Pawpaw hybrid
« on: September 06, 2020, 03:35:00 PM »
Those plants look extremely healthy. Keep us updated! Great progress
Thanks for your feedback. Yes I will keep you informed as well. There would already be a lot more to tell. For example, the difference in the seedlings - this could perhaps be an indication of differences in the chromosome sets. According to the studies shown in the above-mentioned postscript that seedlings of hybrids can have different chromosome sets in different proportions,. Then I could show pictures of the seeds, which actually looked very strange. Soon I will harvest a pawpaw fruit that was pollinated with cherimoya pollen, I will definitely take pictures of the seeds and document all interesting observations about the future seedlings.

22
Temperate Fruit Discussion / Re: Cherimoya/Pawpaw hybrid
« on: September 05, 2020, 04:13:33 PM »
@ Guanabanus
Very constructive and interesting - your report on the hybrid you made - thank you for this Information.


Yes absolutely, a polyploid hybrid does not have to be fertile.

Also there are examples which show that polyploidy can help to get fertile plant  hybrids. The cereale hybrid Triticiale - a cross between wheat (Triticum aestivum L.) as the female and rye (Secale cereale L.) as the male partner (×Triticosecale or Triticosecale Wittmack) ) the chromosome sets were doubled by using colchicine in the seedlings to obtain fertile offspring. In other words, the doubling of the chromosome sets could only lead to reproductive ability. Triticiale offsprings are usually selsbstungen from Trticicale x Triticiale h itself would actually have the possibility to work with colhizin, but I have not yet worked with it. I would have to read in and study the method, then I could also use it on the Annonaceae.

At this point I like to remember to one of my last posts - to the presentation which takes a summary of

Haldan's rule and the hybridogenic speciation and postzygotic incompatibility. Haldane (1892-1964) was a British Indian geneticist.

https://www.kunz.hhu.de/fileadmin/redaktion/Fakultaeten/Mathematisch-Naturwissenschaftliche_Fakultaet/Biologie/Institute/weitere_und_ehemalige_Dozenten/Prof._Dr._Kunz/07_Haldane_hybridogene_Artbildung.ppt (Sorry that it is written in German language. But the pictures will help to explain.)

So in genetics everything is possible. From no result to infertility to the emergence of a separate species with differentiation by developing an incompatibility to backcrossing with the parents. In the plant kingdom the development of incompatible hybrids

At this point I would also like to refer to another interesting report and quote from it: I think some of you, you maybe anyway, I will already know, A. muricata is also mentioned, sorry not A. coriacea )

Polyploidy in Fruit Tree Crops of the Genus Annona (Annonaceae)
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378316/
https://www.researchgate.net/publication/331019625_Polyploidy_in_Fruit_Tree_Crops_of_the_Genus_Annona_Annonaceae/fulltext/5c6181d692851c48a9ca964d/Polyploidy-in-Fruit-Tree-Crops-of-the-Genus-Annona-Annonaceae.pdf
"Genome duplication or polyploidy is one of the main factors of speciation in plants. It is especially frequent in hybrids and very valuable in many crops. (...) Surprisingly, while the hybrid atemoya has been reported as diploid, flow cytometry analysis of a progeny obtained from an interspecific cross between A. cherimola and A. squamosa showed an unusual ploidy variability that was also confirmed karyotype analysis. hile the progeny from intraspecific crosses of A. cherimola showed polyploid genotypes that ranged from 2.5 to 33%, the hybrid atemoyas from the interspecific cross showed 35% of triploids from a total of 186 genotypes analyzed. With the aim of understanding the possible implications of the production of non-reduced gametes, pollen performance, pollen size and frequency distribution of pollen grains was quantified in the progeny of this cross and the parents. A large polymorphism in pollen grain size was found within the interspecific progeny with higher production of unreduced pollen in triploids (38%) than in diploids (29%). Moreover, using PCR amplification of selected microsatellite loci, while 13.7% of the pollen grains from the diploids showed two alleles, 41.28% of the grains from the triploids amplified two alleles and 5.63% showed up to three alleles. This suggests that the larger pollen grains could correspond to diploid and, in a lower frequency, to triploid pollen. Pollen performance was also affected with lower pollen germination in the hybrid triploids than in both diploid parents. The results confirm a higher percentage of polyploids in the interspecific cross, affecting pollen grain size and pollen performance. The occurrence of unreduced gametes in A. cherimola, A. squamosa and their interspecific progeny that may result in abnormalities of ploidy such as the triploids and tetraploids observed in this study, opens an interesting opportunity to study polyploidy in Annonaceae.
(...)
Polyploidy is believed to be a major mechanism of adaptation and speciation, recognized as a major force in evolution (Van de Peer et al., 2017) and very valuable for crop improvement (Udall and Wendel, 2006; Mason, 2016). Polyploidy is more common in plants than in animals. It is estimated that between 30 and 70% of extant flowering plant species are polyploids (...)"

More I will not paste here. The best is to study the complete report by yourself!

Bytheway...to my new hybridizations of this year there is again a small setback. One of the ovaries, which took weeks to show that pollination was successful, has now decided to dry out after some growth. Another one is still in good shape, I hope it stays that way. Last year, such an ovary grew into a strongly deformed fruit, which is another sign of an unusual way of embryo formation. Unfortunately this fruit dried up in centimeter size and still hangs on the tree like a mummy. I have pictures, but I do not want to overload this post!

23
Temperate Fruit Discussion / Re: Cherimoya/Pawpaw hybrid
« on: September 05, 2020, 04:11:18 PM »
@ Guanabanus
Very constructive and interesting - your report on the hybrid you made - thank you for this Information!.


Yes absolutely, a polyploid hybrid does not have to be fertile.

Also there are examples which show that polyploidy can help to get fertile plant  hybrids. The cereale hybrid Triticiale - a cross between wheat (Triticum aestivum L.) as the female and rye (Secale cereale L.) as the male partner (×Triticosecale or Triticosecale Wittmack) ) the chromosome sets were doubled by using colchicine in the seedlings to obtain fertile offspring. In other words, the doubling of the chromosome sets could only lead to reproductive ability. Triticiale offsprings are usually selsbstungen from Trticicale x Triticiale h itself would actually have the possibility to work with colhizin, but I have not yet worked with it. I would have to read in and study the method, then I could also use it on the Annonaceae.

At this point I like to remember to one of my last posts - to the presentation which takes a summary of

Haldan's rule and the hybridogenic speciation and postzygotic incompatibility. Haldane (1892-1964) was a British Indian geneticist.

https://www.kunz.hhu.de/fileadmin/redaktion/Fakultaeten/Mathematisch-Naturwissenschaftliche_Fakultaet/Biologie/Institute/weitere_und_ehemalige_Dozenten/Prof._Dr._Kunz/07_Haldane_hybridogene_Artbildung.ppt (Sorry that it is written in German language. But the pictures will help to explain.)

So in genetics everything is possible. From no result to infertility to the emergence of a separate species with differentiation by developing an incompatibility to backcrossing with the parents. In the plant kingdom the development of incompatible hybrids

At this point I would also like to refer to another interesting report and quote from it: I think some of you, you maybe anyway, I will already know, A. muricata is also mentioned, sorry not A. coriacea )

Polyploidy in Fruit Tree Crops of the Genus Annona (Annonaceae)
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378316/
https://www.researchgate.net/publication/331019625_Polyploidy_in_Fruit_Tree_Crops_of_the_Genus_Annona_Annonaceae/fulltext/5c6181d692851c48a9ca964d/Polyploidy-in-Fruit-Tree-Crops-of-the-Genus-Annona-Annonaceae.pdf
"Genome duplication or polyploidy is one of the main factors of speciation in plants. It is especially frequent in hybrids and very valuable in many crops. (...) Surprisingly, while the hybrid atemoya has been reported as diploid, flow cytometry analysis of a progeny obtained from an interspecific cross between A. cherimola and A. squamosa showed an unusual ploidy variability that was also confirmed karyotype analysis. hile the progeny from intraspecific crosses of A. cherimola showed polyploid genotypes that ranged from 2.5 to 33%, the hybrid atemoyas from the interspecific cross showed 35% of triploids from a total of 186 genotypes analyzed. With the aim of understanding the possible implications of the production of non-reduced gametes, pollen performance, pollen size and frequency distribution of pollen grains was quantified in the progeny of this cross and the parents. A large polymorphism in pollen grain size was found within the interspecific progeny with higher production of unreduced pollen in triploids (38%) than in diploids (29%). Moreover, using PCR amplification of selected microsatellite loci, while 13.7% of the pollen grains from the diploids showed two alleles, 41.28% of the grains from the triploids amplified two alleles and 5.63% showed up to three alleles. This suggests that the larger pollen grains could correspond to diploid and, in a lower frequency, to triploid pollen. Pollen performance was also affected with lower pollen germination in the hybrid triploids than in both diploid parents. The results confirm a higher percentage of polyploids in the interspecific cross, affecting pollen grain size and pollen performance. The occurrence of unreduced gametes in A. cherimola, A. squamosa and their interspecific progeny that may result in abnormalities of ploidy such as the triploids and tetraploids observed in this study, opens an interesting opportunity to study polyploidy in Annonaceae.
(...)
Polyploidy is believed to be a major mechanism of adaptation and speciation, recognized as a major force in evolution (Van de Peer et al., 2017) and very valuable for crop improvement (Udall and Wendel, 2006; Mason, 2016). Polyploidy is more common in plants than in animals. It is estimated that between 30 and 70% of extant flowering plant species are polyploids (...)"

More I will not paste here. The best is to study the complete report by yourself!

Bytheway...to my new hybridizations of this year there is again a small setback. One of the ovaries, which took weeks to show that pollination was successful, has now decided to dry out after some growth. Another one is still in good shape, I hope it stays that way. Last year, such an ovary grew into a strongly deformed fruit, which is another sign of an unusual way of embryo formation. Unfortunately this fruit dried up in centimeter size and still hangs on the tree like a mummy. I have pictures, but I do not want to overload this post!

24
Temperate Fruit Discussion / Re: Cherimoya/Pawpaw hybrid
« on: September 05, 2020, 02:17:07 AM »
keep us posted
Yes I will share any news here. Soon I will harvest the first Pawpaw fruit which flower has polinated with Cherimoya pollen. From this fruit and fromthe seeds I will share pictures.

Is there a way to genetically test the leaf? Maybe some university nearby that has something that could count the chromosomes?
Certainly there are institutes and universities here that could carry out a genetic analysis. Years ago, I wanted to have one done on a very interesting Vitis hybrid, which I created by open pollination, and I have contacted several institutions. Most of them refused, because they are busy with their own work, and the few who would have done it, demanded unreasonably much money. So I prefer to wait until I can see from other characteristics whether I have achieved hybrids or not. And if it worked, the chromosome set has to be doubled, because of the different chromosome sets of Cherimoya and Pawpaw.

This is super exciting stuff. Thank you for doing this experiment.
Thank you - you are welcome! I have to admit, it is also fun and all objections, comments and suggestions are inspiring and absolutely valuable for the evaluation and further development of this experiment!

That delay in fruit-set development is very interesting!

I don't recall having observed that.

I did sometimes observe the opposite, where foreign pollinations would be rejected so fast that there seemed to be an allergic reaction.
Interesting is also your observation and I think this does not contradict my observation, because the majority of Cherimoya flowers pollinated with Pawpaw pollen reacts in principle as u wrote, a very short time passes, mostly even shorter than if the flowers had not been pollinated, and the flower dries up and dies, The death of the flower is different and may give an indication whether perhaps genetic processes have already started or not. Some flowers dry out very quickly and evenly, i.e. petals and base of fruit, and some flowers first let the three petals dry out - like a dry mummy, which often remains loose at the base of fruit - and then dry out later with a little delay.



25
Temperate Fruit Discussion / Re: Cherimoya/Pawpaw hybrid
« on: September 04, 2020, 05:05:33 PM »
@TrilobaTracker and @Guanabanus- Thank you for your contributions and thoughts. Well, I have excluded 99,9% of external influences, because on the one hand the flowers have bloomed single and on the other hand I have protected them against possible cross-pollination by insects with a microporous bag. If self-pollination has occurred, it is something I had no influence on, and I do not want to exclude it, and that is - if the seedlings are not hybrids - with 99,9% probability. But what makes me positive is that by varying many parameters I was able to optimize - beginning by the selection of the optimal pollen donor for pollination up up to more and more refinement of the process steps and boundary conditions to achieve the greatest possible probable pollination success, and this reproducible. This means, that there are many important things you have to pay no attention to, and here the right time of pollination is the least problem. Then I also noticed that there is a clear difference between pollinating Cherimoya flowers with cherimoya pollen and pollinating Cherimoya flowers with Pawpaw pollen. If Cherimoya flowers are pollinated with Cherimoxya pollen, it takes a few days and you can see that the pollination is successful and the ovary grows. If Cherimoya flowers are pollinated with Pawpaw pollen, it takes weeks and you get the impression that the plant is thinking about whether this was a pollination at all. And during this time most pollinated ovaries fall off. And a few of them, after several weeks, begin to decide to stay on and grow very slowly. This could be an indication of meiotic chromosome duplication without cell division. I can say more if the seedlings are in an older stage. At the same time I am also producing the inverse hybrid on Pawpaw as mother plant and I have also some more this year's fruit sets with pollination by Pawpaw pollen on the Cherimoya mother plant, which are a bit bigger than pinhead size.

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