Citrus > Cold Hardy Citrus
Creating a new citrus chimera like Prague Citsuma
Mulberry0126:
For those interested, I found this fascinating thesis study where the researcher was able to produce chimeric shoots from the grafting union between citrus seedlings. I think this method could be reproduced to some degree with trifoliate orange and other (non-trifoliate hybrid) cold hardy citrus like Yuzu, Keraji, etc.
This could potentially allow for a greater diversity of chimeras that are hardy to at least 0°F and push citrus much further North. Even if done with less hardy citrus like lemons and limes, it could still make it possible to grow these fruit in otherwise impossible locations.
It's just a thought right now, but I plan on experimenting with some of my seedlings to see if I can produce a chimera.
Here's the link to the study:
INTERVARIETAL CHIMERA FORMATION BY GRAFTING IN CITRUS By YILING ZHOU ... https://ufdcimages.uflib.ufl.edu/UF/E0/05/04/92/00001/ZHOU_Y.pdf
drymifolia:
It does sound interesting, but their results table is discouraging. Out of more than 1500 seedlings, they only successfully got 8 chimeral shoots, all of which were on the same combination of White mandarin and Moro orange:
I agree that trying to create chimeras is a great idea, but I'd want a method with slightly higher success rates before I expend much effort emulating it.
I do see that they didn't test 100% of the adventitious shoots, so there could have been other chimeras they didn't identify among those, but they specifically selected the ones they thought were most likely to be chimeras based on their placement, so I wouldn't count on there being too many more than the 8 they confirmed.
Mulberry0126:
--- Quote from: drymifolia on December 31, 2023, 11:48:19 AM ---It does sound interesting, but their results table is discouraging. Out of more than 1500 seedlings, they only successfully got 8 chimeral shoots, all of which were on the same combination of White mandarin and Moro orange:
I agree that trying to create chimeras is a great idea, but I'd want a method with slightly higher success rates before I expend much effort emulating it.
I do see that they didn't test 100% of the adventitious shoots, so there could have been other chimeras they didn't identify among those, but they specifically selected the ones they thought were most likely to be chimeras based on their placement, so I wouldn't count on there being too many more than the 8 they confirmed.
--- End quote ---
That's true, there's no guarantee any of the desired combinations could produce a chimera, let alone a stable one. I have a feeling citrus with more similar ancestry may play nicer than more different ones like kumquat+ grapefruit.
sc4001992:
This is pretty good info. To get 1/188 chimera from grafting is not bad at all. But if you use the "improved Ohtsu‟s grafting method" he got 1/21 chimera which is a very good number.
Anyone that knows how to micrograft can play with these techniques. I have so many seedlings I think I will try this same experiment myself. I have the Hamlin and Natsudadi so it would be interesting to see if I can get any chimera from 200 grafts using the improved DHS method.
Thanks for the article, now I have something else to try for next year.
sc4001992:
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